E Tests

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Verotoxogenic E.coli see Haemorrhagic uraemic syndrome

 

Ear infections (otitis media, otitis externa)

 

  • Test Name: Ear infections (otitis media, otitis externa)
  • Laboratory: Microbiology
  • Specimen Type: Swab
  • Specimen Container: Amies swab
  • Specimen Volume: N/A
  • Specimen Requirements: Refrigerate if delivery to the laboratory is delayed
  • Other details:N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: 2-4 days
  • Phone alert limits: Beta Haemolytic Streptococcus Group A isolates, Beta Haemolytic Streptococcus Group B isolates from maternity, post natal and neonatal patients, new MRSA and VRE isolates from inpatients are all phoned to the requesting clinician
  • Preanalytical stability at room temp:N/A

 

Ebstein Barr Virus (EBV) Serology

 

  • Test Name: Ebstein Barr Virus (EBV)
  • Laboratory: Serology
  • Specimen Type: Clotted Blood Specimen
  • Specimen Container: Yellow, white (paediatric).
  • Specimen Volume: 4ml clotted blood (1.5ml paediatric).
  • Specimen Requirements: N/A
  • Other details: N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: 1 day.
  • Phone alert limits:N/A
  • Preanalytical stability at room temp: N/A

 

Ebstein Barr Virus (EBV) PCR

  • Test Name: Ebstein Barr Virus (EBV) PCR
  • Laboratory: Serology External
  • Specimen Type: EDTA Blood Specimen
  • Specimen Container: Purple, red (paediatric).
  • Specimen Volume: 2.5ml clotted blood (1.5ml paediatric).
  • Specimen Requirements: Refrigerate if delivery to the laboratory is delayed.
  • Other details: N/A
  • Frequency of analysis/ assay: Referred to external test site.
  • Turnaround Time: 7-10 days.
  • Phone alert limits: First positive phoned to the requesting clinician.
  • Preanalytical stability at room temp: To be received in Serology Laboratory on day of collection - requires freezing at -20C.

eGFR -Estimated Glomerular Filtration Rate

  • Test Name: eGFR: CKD-EPI
  • Laboratory: Biochemistry
  • Specimen Type: Serum/Plasma
  • Specimen Container: Yellow (Green for UHW in house samples only)
  • Specimen Volume: 4mls
  • Specimen Requirements: eGFR are calculated on all creatinine results for patients >18yrs age
  • Other details: eGFR is an estimate only. Creatinine must be stable (may be unreliable in acutely ill patients). Not valid in pregnancy, children or at extremes of body type.
  • Frequency of analysis/assay: Daily
  • Turnaround Time: Routine-2 working day. Urgent-< 3hrs
  • Phone Alerts: N/A

  • Preanalytical stability at room temp: N/A

     

    Electrolytes - Serum (Sodium and Potassium)

  • Test Name: Electrolytes - Serum (Sodium and Potassium)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow (Green for UHW in house samples only)
  • Specimen Volume: 4mls
  • Specimen Requirements: Overnight unspun specimens are unsuitable
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: Routine - 2 working day Urgent <3 hours
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Electrolytes - Urinary (Sodium and Potassium)

 

  • Test Name: Electrolytes - Urinary (Sodium and Potassium)
  • Laboratory: Biochemistry
  • Specimen Type: Random urine or 24 hour urine
  • Specimen Container: Sarstedt Urine Z bottle (yellow cap)  or 24 hour urine container.
  • Specimen Volume: 3.2 mls or complete collection
  • Specimen Requirements: Boric acid conatiner (red top) unsuitable.
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: 2 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Electron Microscopy ( Faeces & Fluids )

 

  • Test Name: Electron Microscopy ( Faeces & Fluids )
  • Laboratory: Serology External
  • Specimen Type: Faeces/Fluids
  • Specimen Container: Sterile universal container.
  • Specimen Volume: 1-2g (spoonful). At least 1ml of fluid.
  • Specimen Requirements: N/A
  • Other details: N/A
  • Frequency of analysis/ assay: Referred to external test site
  • Turnaround Time: 7-10 days
  • Phone alert limits:N/A
  • Preanalytical stability at room temp: N/A

 

Electrophoresis (Albumin, alpha1, alpha 2, beta and
gamma globulin)

 

  • Test Name: Electrophoresis (Albumin, alpha1, alpha 2, beta and gamma globulin)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4mls
  • Specimen Requirements: Plasma specimen is unsuitable
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: 5 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Endocarditis see also Blood Culture

 

  • Test Name: Endocarditis see also Blood Culture
  • Laboratory: Microbiology
  • Specimen Type: Blood Culture, Valve tissue
  • Specimen Container: Blood culture bottle.
  • Specimen Volume: Blood Culture -5-10ml (1-4ml paediatric) /As large a tissue as possible should be sent
  • Specimen Requirements: In suspected endocarditis, three sets of blood cultures should be taken from separate venepuncture sites.
  • Other details: N/A
  • Frequency of analysis/ assay: N/A
  • Turnaround Time: Cultured for 7 days, Identification and susceptibility within 2 days of growth.
  • Phone alert limits:N/A
  • Preanalytical stability at room temp: N/A

 

Enteric Fever see Blood Cultures

 

Enterobius vermicularis (threadworm/pinworm)

  • Test Name: Enterobius vermicularis (threadworm/pinworm)
  • Laboratory: Microbiology
  • Specimen Type: Sellotape slide
  • Specimen Container: Glass slide placed in slide holder for transport
  • Specimen Volume: Recommended to take up to 5 specimens
  • Specimen Requirements: See sellotape slide in ova and parasite section
  • Other details: Glass slide is taken in the morning on rising, before defecation or bathing.
  • Frequency of analysis/ assay:As required
  • Turnaround Time: 1-3 days
  • Phone alert limits:Positives phoned to requesting clinician.
  • Preanalytical stability at room temp:N/A

 

Enterovirus PCR

  • Test Name: Enterovirus PCR (see also meningitis viral)
  • Laboratory: Serology External
  • Specimen Type: Viral (pink topped) swab, faeces specimen
  • Specimen Container: Viral swab (pink topped), faeces specimen in sterile universal container
  • Specimen Volume: 1.2g (spoonful) for faeces specimen
  • Specimen Requirements: Refrigerate if delivery to the lab is delayed
  • Other details: N/A
  • Frequency of analysis/ assay:Referred to external test site
  • Turnaround Time: 7-10 days
  • Phone alert limits:N/A
  • Preanalytical stability at room temp:N/A

Epanutin (Phenytoin)

 

  • Test Name: Epanutin (Phenytoin)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4mls
  • Specimen Requirements:
  • Other details:
  • Frequency of analysis/ assay:
  • Turnaround Time: 2 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Epilim (Valproic acid/sodium valporate)

 

  • Test Name: Epilim (Valproic acid/sodium valporate)
  • Laboratory: Biochemistry
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4mls
  • Specimen Requirements:
  • Other details: Serum concentrations are no better a guide to clinical response than is the dose, therefore routine monitoring of valporate concentrations is not recommended. The measurement in psychiatric patients may be useful when compliance is an issue.
  • Frequency of analysis/ assay:
  • Turnaround Time: 2 working days
  • Phone alert limits:
  • Preanalytical stability at room temp:

 

Erythrocyte Sedimentation Rate (ESR)

 

  • Test Name: Erythrocyte Sedimentation Rate (ESR)
  • Laboratory: Haematology
  • Specimen Type: EDTA
  • Specimen Container: Purple
  • Specimen Volume: 2.5ml
  • Specimen Requirements: Can be performed on same sample as FBC
  • Other details: When both an ESR and CRP are requested the CRP only is analysed; unless it is stated on the lab request form that the ESR is required for a particular clinical reason.
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: Routine - 2 working day
  • Phone alert limits:
  • Preanalytical stability at room temp: Samples should ideally be processed within 4 hours of collection. Please send to the laboratory ASAP.

 

Erythropoietin

 

  • Test Name: Erythropoietin
  • Laboratory: Haematology referral test
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4ml
  • Specimen Requirements: 
  • Other details: Sample to be taken in the morning.
  • Frequency of analysis/ assay: As required
  • Turnaround Time: 10 days
  • Phone alert limits: N/A
  • Preanalytical stability: 7 days at 2 - 8°C

 

Extractable nuclear antigens

 

  • Test Name: Extractable nuclear antigens
  • Laboratory: Autoantibody Lab.
  • Specimen Type: Serum
  • Specimen Container: Yellow
  • Specimen Volume: 4 mls
  • Specimen Requirements: Refrigerate if delivery to the lab is delayed
  • Other details: ENA profile test (including SSA, SSB, Sm, RNP, Jo-1, Scl-70) is requested on all positive ENA screens
  • Frequency of analysis/ assay: Screening test carried out weekly
  • Turnaround Time: 10 days
  • Phone alert limits: N/A
  • Preanalytical stability at room temp: N/A

 

Eye infections (bacterial)

 

  • Test Name: Eye infections
  • Laboratory: Microbiology
  • Specimen Type: Swabs - Conjunctival / Eye, Corneal scraping, Contact lens / fluid
  • Specimen Container: Amies swab, sterile universal container.
  • Specimen Volume: At least 1ml of fluid
  • Specimen Requirements: Refrigerate if delay in delivery to the laboratory. Corneal Scraping innoculated directly onto culture plates/ slides and sent to laboratory
  • Other details: N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: Microscopy same day 2-4 days for culture
  • Phone alert limits:Beta Haemolytic Streptococcus Group A isolates, Beta Haemolytic Streptococcus Group B isolates from maternity, post natal and neonatal patients, new MRSA and VRE isolates from inpatients are all phoned to the requesting clinician
  • Preanalytical stability at room temp: N/A

 

Eye infections (viral)

  • Test Name: Eye infections
  • Laboratory: Serology External
  • Specimen Type: Swabs - Viral swab (pink topped)- Conjunctival / Eye, Corneal scraping, Contact lens / fluid
  • Specimen Container: Pink topped viral swab, sterile universal container.
  • Specimen Volume: At least 1ml of fluid
  • Specimen Requirements: Refrigerate if delay in delivery to the laboratory. 
  • Other details: N/A
  • Frequency of analysis/ assay: Daily
  • Turnaround Time: 7-10 days
  • Phone alert limits: Positive findings are phoned to the requesting clinician
  • Preanalytical stability at room temp: N/A

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